Presence of Chlamydia trachomatis and Mycoplasma spp., but not Neisseria gonorrhoeae and Treponema pallidum, in women undergoing an infertility evaluation: high prevalence of tetracycline resistance gene tet(M)

AMB Express

Table 1 Oligonucleotide primers used in this study

PCR	Target	Primer/probe	Sequence (5′–3′)	Size (bp)	Ref
Generic for 11 Chlamydia species	23S rRNA	UP1	GGGGTTGTAGGRTTGRGGAWAAAGGATC	168	Guo et al. (2016)
		UP2	GGGGTTGTAGGGTCGATAAYATGRGATC
		DN	GAGAGTGGTCTCCCCAGATTCARACTA
		FLU1	ACGAAAGGAGAKMAAGACYGACCTCAAC-6-FAM
		FLU2	ACGAAAAAACAAGAGACTCTATTCGAT-6-FAM
		LCRed	LCRed640-CCTGAGTAGRRCTAGACACGTGAAAC-P
Generic for Mycoplasma species	16S rRNA	UP	CTGCCTGAGTAGTAYRYTCGCAA	174	This study
		DN	TGCACCATCTGTCACTHBGTTARCCTC
		FLU	AAACCACATGCTCCACCGCTTGT-36-FAM
		LCRed	LCRed640-GGTCCCCGTCAATTCCTTTAAGTTT-P
Mycoplasma species for sequencing	16S rRNA	UP	ACTCCTACGGGAGGCAGCAGTA	700	Yoshida et al. (2002)
Mycoplasma species for sequencing	16S rRNA	DN	TGCACCATCTGTCACTCTGTTAACCTC	700	Yoshida et al. (2002)
N. gonorrhoeae	porA pseudogene	UP	CGGTTTCCGTGCGTTACGA	132	Whiley et al. (2004)
		DN	AACTGGTTTCATCTGATTACTTTCCA
		FLU	CATTCAATTTGTTCCGAGTCAAAACAGC-6-FAM
		LCRed	LCRed640-AGTCCGCCTATACGCCTGCTACTTTCAC-P
T. pallidum	polA	UP	GGTAGAAGGGAGGGCTAGTA	104	Heymans et al. (2010)
		DN	CTAAGATCTCTATTTTCTATAGGTATGG
		TaqMan probe	FAM-ACACAGCACTCGTCTTCAACTCC-BHQ1
RPPs gene		UP	CCACCGAATCCTTTCTGGGC	245	This study
RPPs gene		DN	ATCCGAAAATCTGCTGGGGTACT	245	This study