From: Evaluation of microbial consortia and chemical changes in spontaneous maize bran fermentation
Primers | Target | Sequence (5′–3′) | Thermal conditions | Amplicon (bp) | References |
---|---|---|---|---|---|
RSA_F RSA_R | 16S–23S rDNA spacer region (RSA) | GAAGTCGTAACAAGG CAAGGCATCCACCGT | 94 °C × 45 s 54 °C × 1 min × 35 cycles | Variable | Lane (1991) |
16S_F 16S_R | 16S rDNA gene | AGAGTTTGATCCTGGCTCAG CTACGGCTACCTTGTTACGA | 94 °C × 45 s 55 °C × 45 s × 35 cycles | 1540 | Lane (1991) |
p8FLP p806R | Partial 16S rDNA gene | AGTTTGATCCTGGCTCAG GGACTACCAGGGTATCTAAT | 94 °C × 1 min 56 °C × 1 min × 30 cycles | 800 | McCabe et al. (1995) |
L. plant_F L. plant_R | L. plantarum | CCGTTTATGCGGAACACC TCGGGATTACCAAACATCAC | 94 °C × 2 min 56 °C × 1 min × 35 cycles | 318 | Torriani et al. (2001) |
Pedio23S_F Pedio23S_R | Pediococcus spp. | GAACTCGTGTACGTTGAAAAGTGCTGA GCGTCCCTCCATTGTTCAAACAAG | 94 °C × 45 s 64 °C × 1 min × 35 cycles | 701 | Pfannebecker and Fröhlich (2008) |
P23S_R PPE23S_F | P. pentosaceus | CTGTCTCGCAGTCAAGCTC CCAGGTTGAAGGTGCAGTAAAAT | 94 °C × 1 min 67 °C × 1 min × 35 cycles | 1640 | Pfannebecker and Fröhlich (2008) |
P23S-R ArgentF | P. argentinicus | CTGTCTCGCAGTCAAGCTC GATATTCCTGTACTAGTTAGAT | 94 °C × 1 min 60 °C × 1 min × 35 cycles | 948 | This study |
Lbrev 1391F | L. brevis | TAATGATGACCTTGCGGTC TGTACACACCGCCCGTC | 94 °C × 45 s 48 °C × 45 s × 35 cycles | 330 | Coton et al. (2008) |
Weiss_F Weiss_R | Weissella spp. | CGTGGGAAACCTACCTCTTA CCCTCAAACATCTAGCAC | 94 °C × 45 s 54 °C × 1 min × 35 cycles | 725 | Jang et al. (2002) |
1 RL LaccreR | L. lactis | TTTGAGAGTTTGATCCTGG GGGATCATCTTTGAGTGAT | 94 °C × 2 min 54 °C × 1 min × 35 cycles | 238 | Pu et al. (2002) |
LeuclacF LeuclacR | L. lactis | AGGCGGCTTACTGGACAAC CTTAGACGGCTCCTTCCAT | 94 °C × 2 min 58 °C × 1 min × 35 cycles | 742 | Lee et al. (2000) |
ITS1 ITS4 | ITS1-5.8S-ITS2 Internal Transcribed Spacer (ITS) | TCCGTAGGTGAACCTGCGG TCCTCCGCTTATTGATATGC | 94 °C × 45 s 60 °C × 1 min × 35 cycles | Variable | Jespersen et al. (2005) |
NL1 NL4 | 26S rDNA gene | GCATATCAATAAGCGGAGGAAAAG GGTCCGTGTTTCAAGACGG | 94 °C × 2 min 52 °C × 1 min × 35 cycles | 778 | Kurtzman and Robnett (1998) |