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Table 2 Thermal conditions used in this method

From: Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites

Steps Amplification style Thermal condition Dilution (fold)
1 Linear 98 °C 10 s, 62 °C 5 s, 72 °C 30 s; 20 cycles 100
Exponential 98 °C 10 s, 25 °C 5 s, 72 °C 30 s; 1 cycle
98 °C 10 s, 58 °C 5 s, 72 °C 30 s; 18 cycles
100
2 Linear 98 °C 10 s, 62 °C 5 s, 72 °C 30 s; 20 cycles 10
Exponential 98 °C 10 s, 68 °C 5 s, 72 °C 30 s, 98 °C 10 s, 63 °C 5 s, 72 °C 30 s, 98 °C 10 s, 50 °C 5 s, 72 °C 30 s; 7 cycles 100
3 Exponential 98 °C 10 s, 68 °C 5 s, 72 °C 30 s, 98 °C 10 s, 63 °C 5 s, 72 °C 30 s, 98 °C 10 s, 50 °C 5 s, 72 °C 30 s; 13 cycles