Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites

AMB Express

Table 2 Thermal conditions used in this method

Steps	Amplification style	Thermal condition	Dilution (fold)
1	Linear	98 °C 10 s, 62 °C 5 s, 72 °C 30 s; 20 cycles	100
1	Exponential	98 °C 10 s, 25 °C 5 s, 72 °C 30 s; 1 cycle 98 °C 10 s, 58 °C 5 s, 72 °C 30 s; 18 cycles	100
2	Linear	98 °C 10 s, 62 °C 5 s, 72 °C 30 s; 20 cycles	10
2	Exponential	98 °C 10 s, 68 °C 5 s, 72 °C 30 s, 98 °C 10 s, 63 °C 5 s, 72 °C 30 s, 98 °C 10 s, 50 °C 5 s, 72 °C 30 s; 7 cycles	100
3	Exponential	98 °C 10 s, 68 °C 5 s, 72 °C 30 s, 98 °C 10 s, 63 °C 5 s, 72 °C 30 s, 98 °C 10 s, 50 °C 5 s, 72 °C 30 s; 13 cycles