Table 1 Primers used in this method
Primer name | Sequence | Tm (°C) | Description |
|---|---|---|---|
F389 | 5′-TCAAGCATTTTATCCGTACTCCTG-3′ | 55.39 | Designed by our lab |
F536 | 5′-CGGTTGCATTCGATTCCTGTTTGTA-3′ | 58.73 | Designed by our lab |
F772 | 5′-TAGGTTGTATTGATGTTGGACGAG-3′ | 55.09 | Designed by our lab |
LAD1 | 5′-ACGATGGACTCCAGAG(G/C/A)N(G/C/A)NNNGGAA-3′ | (Liu and Chen 2007) | |
LAD3 | 5′-ACGATGGACTCCAGAG(T/A/C)N(A/G/C)NNNCCAC-3′ | (Liu and Chen 2007) | |
AC1 | 5′-ACGATGGACTCCAGAG-3′ | 49.15 | (Liu and Chen 2007) |