Table 1 Nucleotide sequences of oligonucleotides designed for assembly of mu-IFN-CSP
From: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
Primers | Nucleotide sequence (from 5′ end to 3′ end) |
|---|---|
MuIC-1 | GGAATTCCATATG TGTGATCTGCCTCAGA |
MuIC-2 | GTGCCAGGAGAATCAAGGCACGACGGTTACCCAGGCTGTGAGTCTGAGGCAGATCACA |
MuIC-3 | GCCTTGATTCTCCTGGCACAAATGCGTCGTATCTCTCCTTTCTCCTGCCTGAAGGACC |
MuIC-4 | TATCATCAAACTCCTCCTGTGGGAATTCAAAGTCATGACGGTCCTTCAGGCAGGAGAA |
MuIC-5 | CAGGAGGAGTTTGATGATAAACAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTCCATG |
MuIC-6 | CTTTTGTGGTAAAGAGGTTGAAGATCTGCTGGATCATCTCATGGAGGACAGAGATGGC |
MuIC-7 | AACCTCTTTACCACAAAAGATTCATCTGCTGCTTGGGATGAGGACCTCCTTGACAAAT |
MuIC-8 | CTTCCAAGTCATTCAGCTGCTGGTAGAGTTCGGTGCAGAATTTGTCAAGGAGGTCCTC |
MuIC-9 | CAGCTGAATGACTTGGAAGCCTGTGTGATGCAGGAGGAGCGTGTGGGAGAAACTCCAC |
MuIC-10 | AGTATTTCTTCACAGCCAAGATGGAGTCCGCATTCATCAGTGGAGTTTCTCCCACACG |
MuIC-11 | TTGGCTGTGAAGAAATACTTCCGTCGTATCACTCTCTATCTGACAGAGAAGAAATACA |
MuIC-12 | GCATGATTTCTGCACGGACAACCTCCCAGGCACAAGGGCTGTATTTCTTCTCTGTCAG |
MuIC-13 | GTCCGTGCAGAAATCATGCGTTCCCTCTCTTTATCAACAAACTTGCAAGAACGTTTAC |
MuIC-14 | CGTAATTTCTCGTTGTCTTCCTTACGACGTAAACGTTCTTGCAAGTT |
MuIC-15 | GACAACGAGAAATTACGTAAACCAAAACATAAAAAATTAAAGCAACCAGCGGA |
MuIC-16 | CCGCTCGAGACCATCCGCTGGTTGCTTTAA |