Fig. 6
From: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
Comparison of the solubility and characterization of recombinant protein. a Assay of expression and solubility of IFN-CSP in recombinant E. coli. b Assay of expression and solubility of mu-IFN-CSP in recombinant E. coli before optimization. c Assay of expression and solubility of mu-IFN-CSP in recombinant E. coli after optimization and purification of mu-IFN-CSP. Lane M protein molecular weight marker. Lanes 0–1 total proteins of recombinant E. coli before and after induction. Lanes 2–3 supernatant and precipitation after ultrasonication and centrifugation. Lane 4 eluted protein fraction using HisTrap affinity chromatography. Lane 5 purified mu-IFN-CSP using HisTrap affinity chromatography. The arrows indicate the intense bands at 21.5 kDa corresponding to the target protein. d Analysis of purified IFN-CSP by RP-HPLC with a C18 column