Fig. 4
From: Production of bioactive liver-targeting interferon Mu-IFN-CSP by soluble prokaryotic expression
Schematic diagram of mu-IFN-CSP gene in the expression vector mu-IFN-CSP/pET-21b and expression of mu-IFN-CSP protein in recombinant E. coli. a A schematic diagram of mu-IFN-CSP/pET-21b. b SDS-PAGE analysis of mu-IFN-CSP expression. Lane 1 total proteins of recombinant E. coli BL21 after IPTG induction. Lanes 2–3 supernatant and precipitation after ultrasonication and centrifugation. Lane M protein molecular weight marker. c Recombinant mu-IFN-CSP was analyzed by western blot analysis. Lanes 1–2 total proteins of E. coli BL21/pET-21b-mu-IFN-CSP before and after induction. Lane M protein molecular weight marker