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AMB Express

Table 2 Kinetic parameters of the wild-type and W361F variant enzymes for ginsenosides Rb1 and Rd

From: Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase

Enzyme

Substrate

K m (mM)

k cat (s−1)

k cat/K m (s−1 mM−1)

Wild-type

Rb1

0.57 ± 0.01

2257 ± 33

3967 ± 91

Rd

0.52 ± 0.01

92 ± 1

178 ± 4

W361F

Rb1

1.93 ± 0.02

1343 ± 28

697 ± 16

Rd

1.59 ± 0.02

1049 ± 12

660 ± 11

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