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Table 2 Kinetic parameters of the wild-type and W361F variant enzymes for ginsenosides Rb1 and Rd

From: Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase

Enzyme Substrate K m (mM) k cat (s−1) k cat/K m (s−1 mM−1)
Wild-type Rb1 0.57 ± 0.01 2257 ± 33 3967 ± 91
Rd 0.52 ± 0.01 92 ± 1 178 ± 4
W361F Rb1 1.93 ± 0.02 1343 ± 28 697 ± 16
Rd 1.59 ± 0.02 1049 ± 12 660 ± 11