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Table 1 Specific activities of the wild-type and W361F variant enzymes for ginsenosides and flavanones

From: Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase

Substrate

Specific activity (nmol min−1 mg−1)

Wild-type

W361F

Ginsenoside

 Rb1

5082 ± 7.6

3213 ± 10.6

 Rb2

1643 ± 3.7

9077 ± 15.9

 Rc

20.1 ± 0.6

23.3 ± 1.1

 Rd

338 ± 1.2

1415 ± 3.8

 F2

13,130 ± 20.5

13,227 ± 42.3

 Compound O

6354 ± 12.8

2031 ± 5.2

 Compound Y

4588 ± 10.1

1347 ± 4.9

 Compound Mc1

1.9 ± 0.2

12.0 ± 0.1

 Compound Mc

15.4 ± 0.2

7.2 ± 0.1

Flavanone

 Hesperidin

39.1 ± 0.3

288 ± 0.6

 Naringin

233 ± 0.3

35.5 ± 0.2