Fig. 4

Hydrolysis from ginsenoside Rb₁ to compound K via Rd and F₂ by the wild-type and W361F-variant SS-BGLs. Ginsenoside F₂ was not detected in the hydrolysis reactions because of high specific activity for ginsenoside F₂. Ginsenoside Rb₁ (closed square), Rd (closed circle), and compound K (open circle). a Using 0.01 mg ml⁻¹ wild-type enzyme. b Using 0.01 mg ml⁻¹ W361F variant enzyme. c Using 0.1 mg ml⁻¹ wild-type enzyme. d Using 0.1 mg ml⁻¹ W361F variant enzyme. Data represent the means from three separate experiments, and the error bars represent standard deviations