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Fig. 6 | AMB Express

Fig. 6

From: LsrB-based and temperature-dependent identification of bacterial AI-2 receptor

Fig. 6

Binding of LsrB to exogenous AI-2. Different concentrations of AI-2 (2.5, 10.0 and 40.0 μM) were incubated with 5 mg/ml of LsrB (BL21∆luxS). The results of AI-2 binding activity indicated that 5 mg/ml of LsrB (BL21∆luxS) could bind exogenous AI-2 from 2.5 to 40 μM. Furthermore, the assay of AI-2 releasing activity showed that bound AI-2 was released from LsrB (BL21∆luxS) at 55 °C for 10 min. However, denatured LsrB (BL21∆luxS) was not able to bind to AI-2

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