Fig. 1
From: LsrB-based and temperature-dependent identification of bacterial AI-2 receptor
Identification of the luxS mutant BL21∆luxS. a Schematic chart of strategy for producing the BL21 luxS deletion mutant. The luxS was deleted by replacing the partial gene sequence of luxS with kanamycin resistance cassette at SalI cleavage sites. The primers used for the confirmation of the luxS deletion are also indicated. b Identification of the luxS mutant BL21∆luxS. Lane M: DL 2000 DNA marker (D501A; Takara); lane 1: the wild-type strain BL21 showed a 2519-bp PCR product using primers LuxS-OutF/LuxS-OutR; lane 2: the mutant BL21∆luxS with cure of the kanamycin resistance cassette showed a 2209-bp PCR product using primers LuxS-OutF/LuxS-OutR; lane 3: negative control; lane 4: the wild-type strain DE17 showed a 307-bp PCR product using primers LuxS-inF/LuxS-inR; lane 5: the mutant DE17∆pfs showed no PCR products using primers LuxS-inF/LuxS-inR; lane 6: negative control