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Fig. 7 | AMB Express

Fig. 7

From: Novel archaeal thermostable cellulases from an oil reservoir metagenome

Fig. 7

Results from ICS analysis of degradation products using enzyme F1 on different substrates. In all cases, reactions were performed in a 1 ml volume containing 50 µl F1 enzyme, i.e. 1:20. A standard mixture (std mix) applied gives peaks for cellohexaose, cellobiose and glucose, indicated in panel: a Cellohexaose used as reaction substrates, with 50 µl (1:20) or 0.1 µl (1:10,000) F1 enzyme. Intermediate product peaks detected in low enzyme amount reaction, from right to left pentamer, tetramer, trimer followed by cellobiose and glucose. b Reduced cellohexaose used as reaction substrate. c Avicel used as reaction substrate (reaction products diluted 1:10 prior to analysis by ICS). d β-glucan used as reaction substrate. For the full chromatogram of β-glucan w/wo F1 added, please refer to Additional file 1: Figure S2

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