Fig. 6
From: Novel archaeal thermostable cellulases from an oil reservoir metagenome
Activity of F1 (in heated, 65 °C, 20 min, crude cell extract) on a CMC and b Avicel as a substrate, detected as fluorescence by the Amplex Ultra Red based on H2O2 formed by glucose oxidase from enzyme reactions generating glucose. Enzyme I indicates endoglucanase (Sigma E2164), Enzyme II indicates cellobiohydrolase (Sigma E6412) and Enzyme III indicates beta-glucosidase (Sigma 49290). For assay using CMC, all enzymes were diluted 5x compared to the Avicel reaction in order to resolve results. Error bars represent standard deviations from three independent measurements