|
Morphological tests
|
|
Cell morphology
|
Rod
|
|
Gram’s reaction
|
G−ve
|
|
Motility
|
Non-motile
|
|
Flagella
|
Absent
|
|
Colony characteristics on selective and differential media
|
|
Nutrient agar
|
White to cream colour colonies
|
|
MacConkey’s agar
|
Pink color colonies
|
|
Eosin methylene blue agar
|
Large mucoid colonies, no metallic sheen
|
|
Salmonella Shigella agar
|
Slight growth, light pink color colonies
|
|
TSI agar slant
|
Yellow colour on butt and slant
|
|
Biochemical tests
|
|
Catalase
|
+
|
|
Oxidase
|
−
|
|
Indole
|
−
|
|
VP
|
+
|
|
MR
|
+
|
|
Citrate utilization
|
+
|
|
H2S production
|
−
|
|
Carbohydrate fermentation tests
|
|
Arabinose
|
+
|
|
Glucose
|
+
|
|
Inositol
|
−
|
|
Lactose
|
+
|
|
Maltose
|
+
|
|
Mannitol
|
+
|
|
Sucrose
|
+
|
