Table 3 Determination of microbial diversity of six different extraction methods using DGGE
DNA extraction method | Average number of bands in each specimen | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
S/no: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
STES buffer and phenol chloroform extraction method (BP1) | 14 | 10 | 06 | 08 | 16 | 11 | 06 | 04 | 04 | 08 |
TN150 buffer and phenol chloroform extraction method (BP2) | 14 | 12 | 07 | 09 | 13 | 12 | 07 | 05 | 08 | 09 |
DNA extraction tissue kit (K) | 15 | 06 | 07 | 10 | 11 | 13 | 07 | 04 | 05 | 06 |
Boiling in distilled water (H1) | 13 | 09 | 05 | 08 | 06 | 08 | 05 | 05 | 08 | 05 |
Boiling in aqueous NaOH (H2) | 14 | 11 | 06 | 11 | 10 | 12 | 07 | 06 | 08 | 07 |
Salting out method (S) | 14 | 09 | 05 | 07 | 10 | 12 | 05 | 05 | 08 | 06 |