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Table 2 DNA quality and average DNA yield obtained using different DNA extraction methods

From: Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method

  Patient/specimen No.
01 02 03 04 05 06 07 08 09 10
1 STES buffer and phenol chloroform extraction method (BP1)
 DNA yield (ng/µl) 48.65 ± 25.24 1747.95 ± 404.25 480.2 ± 359.07 195.25 ± 29.06 93.20 ± 31.25 937.75 ± 255.90 900.30 ± 639.79 1096.9 ± 451.7 226 ± 83.43 69.50 ± 10.89
 A260/280 1.98 ± 0.08 1.80 ± 0.01 1.82 ± 0.06 1.88 ± 0.00 1.99 ± 0.02 1.88 ± 0.01 1.80 ± 0.01 1.82 ± 0.01 1.83 ± 0.02 1.96 ± 0.02
 A260/230 0.87 ± 0.05 2.19 ± 0.07 1.75 ± 0.35 1.27 ± 0.02 1.05 ± 0.27 2.15 ± 0.01 1.82 ± 0.40 2.1 ± 0.11 1.69 ± 0.04 0.77 ± 0.05
2 TN150 buffer and phenol chloroform extraction method (BP2)
 DNA yield (ng/µl) 10.9 ± 1.56 310.05 ± 160.16 340.6 ± 12.45 88.35 ± 27.51 46.20 ± 21.64 274.05 ± 43.20 661.45 ± 500.70 402.25 ± 1.91 90.25 ± 76.44 93.70 ± 42.99
 A260/280 2.07 ± 0.04 1.8 ± 0.03 1.77 ± 0.00 1.77 ± 0.04 1.73 ± 0.15 1.71 ± 0.02 1.86 ± 0.99 1.82 ± 0.01 1.8 ± 0.06 1.84 ± 0.12
 A260/230 0.52 ± 0.01 1.84 ± 0.16 1.49 ± 0.10 1.15 ± 0.30 0.69 ± 0.17 1.57 ± 0.08 1.66 ± 0.32 1.79 ± 0.08 1.3 ± 0.44 1.37 ± 0.36
3 DNeasy blood and tissue kit (K)
 DNA yield (ng/µl) 39.45 ± 21.01 400.55 ± 447.25 698.2 ± 126.99 51.8 ± 28.56 50.75 ± 66.39 152.65 ± 13.08 718.1 ± 608.54 80.95 ± 0.49 316.25 ± 127.77 388.40 ± 120.49
 A260/280 1.87 ± 0.05 1.80 ± 0.01 1.81 ± 0.01 1.78 ± 0.11 1.87 ± 0.17 1.80 ± 0.01 1.82 ± 0.01 1.8 ± 0.01 1.81 ± 0 1.84 ± 0.01
 A260/230 2.1 ± 0.40 1.74 ± 0.66 2.17 ± 0.11 2.19 ± 0.06 1.56 ± 0.00 1.65 ± 0.37 2.15 ± 0.07 1.81 ± 0.11 1.82 ± 0.55 2.15 ± 0.06
4 Heating in distilled water (H1)
 DNA yield (ng/µl) 213.25 ± 46.46 51.65 ± 19.02 260.95 ± 70.36 72.2 ± 36.63 142.45 ± 47.73 277.6 ± 9.76 429.05 ± 4.60 145.6 ± 168.57 97.25 ± 73.89 255.35 ± 109.95
 A260/280 1.22 ± 0.04 1.38 ± 0.06 1.26 ± 0.01 1.56 ± 0.05 1.36 ± 0.17 1.39 ± 0.05 1.41 ± 0.05 1.39 ± 0.05 1.41 ± 0.09 1.74 ± 0.18
 A260/230 0.3 ± 0.04 0.28 ± 0.02 0.43 ± 0.00 0.56 ± 0.07 0.29 ± 0.02 0.32 ± 0.01 0.56 ± 1.01 0.33 ± 0.11 0.25 ± 0.04 0.71 ± 0.36
5 Heating in aqueous NaOH (H2)
 DNA yield (ng/µl) 131 ± 25.74 356.9 ± 189.22 606.95 ± 82.94 185.5 ± 32.81 146.8 ± 40.45 386.15 ± 96.1 184.25 ± 70.22 240.65 ± 189.86 210.05 ± 28.5 208.6 ± 113.14
 A260/280 1.08 ± 0.04 1.37 ± 0.04 1.51 ± 0.08 1.38 ± 0.05 1.15 ± 0.04 1.31 ± 0.08 1.46 ± 0.02 1.18 ± 0.13 1.1 ± 0.01 1.47 ± 0.03
 A260/230 0.25 ± 0.03 0.46 ± 0.04 0.79 ± 0.13 0.48 ± 0.06 0.26 ± 0.07 0.27 ± 0.02 0.57 ± 0.00 0.25 ± 0.08 0.3 ± 0.02 0.39 ± 0.01
6 Salting out method (S)
 DNA yield (ng/µl) 62.2 ± 26.87 1701.8 ± 1046.80 3599.4 ± 1363.87 327.5 ± 286.38 118.45 ± 23.83 581 ± 50.91 3264.4 ± 3135.45 1169.75 ± 582.16 246.85 ± 24.96 1248.4 ± 792.67
 A260/280 1.95 ± 0.04 1.81 ± 0.07 1.81 ± 0.04 1.97 ± 0.13 1.95 ± 0.16 1.78 ± 0.04 1.84 ± 0.06 1.83 ± 0.4 1.83 ± 0.04 1.83 ± 0.11
 A260/230 0.9 ± 0.51 0.61 ± 0.3 0.96 ± 0.01 0.37 ± 0.41 0.15 ± 0.04 0.68 ± 0.03 0.77 ± 0.40 0.48 ± 0.25 0.58 ± 0.11 0.46 ± 0.29