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Fig. 4 | AMB Express

Fig. 4

From: Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method

Fig. 4

A comparison of DGGE profiles of PCR amplified bacterial 16S rRNA gene for the specimen No: 2. DNA was extracted using six different DNA extraction methods. Bacterial fingerprinting profile was based on 30–55% denaturing gradient. “L” lanes represent the in house bacterial reference panel which includes S. aureus, Acinetobacter spp., Group B Streptococcus spp., E. faecalis, and E. coli from top to bottom respectively. Other lanes show bacterial fingerprinting profile of each extraction method in duplicate (a, b) for the specimen No. 2, collected from a subject with a chronic wound

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