Table 1 Samples profile, phenotypic and molecular features and bioremediation analysis of mycobacterial isolates from Iranian ecosystems
Sample profile | Phenotypic features | 16S rRNA analysis | Biodegradation activity | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Isolates | Location (city) Province | Source | pH | Temperature | Opt. Tm | Growth rate | Semi quantitative catalase | Tween 80 hydrolyses | Tolerance of NaCl5% | Pyrazinamidase | Reduction of tellurite potassium | Urease | Runyon group | Similarity (%) | Base pair differences | Identification | |
A (2, 12, 13) | Khorramabad (Lorestan) | River sediment/forest soil | 7 | 8–14 | 35 | R | + | + | + | − | − | + | IV | 99.69 | 4/1285 | M. sacrum like | PAHs/sodium sulfate |
A28 | Ahwaz (Khuzestan) | River sediment | 7.2 | 18 | 35 | R | + | + | + | − | − | + | IV | 99.69 | 4/1285 | M. sacrum like | PAHs/sodium sulfate |
A53 | Fulad Shahr (Isfahan) | Forest soil | 7.4 | 30 | 30 | R | − | + | + | − | − | + | IV | 97.74 | 22/974 | M. flavescens | PAHs/crude oil |
A82 | Dorud (Lorestan) | River sediment | 7.2 | 25 | 30 | R | − | + | + | − | − | + | IV | 97.74 | 22/974 | M. flavescens | PAHs/crude oil |
A (113, 88) | Yazd (Yazd) | Hospital water | 7.4 | 14 | 30 | R | − | + | + | − | − | + | IV | 97.74 | 22/974 | M. flavescens | PAHs/crude oil |
A38 | Shazand (Markazi) | Oil refinery soil | 8.5 | 6 | 35 | R | + | − | + | + | − | − | IV | 100 | 0/1074 | M. conceptionense | Dibenzothiophene |
A55 | Abadan (Khuzestan) | Oil refinery soil | 7.6 | 24 | 25 | R | + | + | + | − | − | + | IV | 99.9 | 1/1074 | M. monacense | PAHs/crude oil/pristan |
A75 | Isfahan (Isfahan) | Porcelain wastewater | 6.5 | 20 | 25 | R | + | + | + | − | − | + | IV | 99.9 | 1/1074 | M. monacense | PAHs/crude oil/pristan |
A6 | Dezful (Khuzestan) | Dam water | 7.6 | 13 | 30 | R | − | + | + | − | + | + | IV | 99.56 | 6/1378 | M. vanbaalenii like | PAHs |
A16 | Arak (Markazi) | Salt Lake sediment | 8 | 4 | 25 | R | − | − | − | + | − | + | IV | 98.22 | 24/1352 | M. gadium like | Sodium sulfate |
A4 | Khorram abad (Lorestan) | Land farm soil | 7 | 14 | 25 | R | + | + | − | − | + | + | IV | 99.48 | 7/1356 | M. tusciae like | PAHs |
A23 | Kish (Hormozgan) | Sea sediment | 7.9 | 20 | 30 | R | + | − | + | − | − | + | IV | 98.1 | 18/916 | M. pallens like | PAHs |
A7 | Isfahan (Isfahan) | Hospital water | 7.6 | 12 | 30 | S | − | − | + | + | − | − | II | 99 | 9/960 | M. cookii like | – |
A15 | Omidie (Khuzestan) | Oil contaminated soil | 6.4 | 25 | 25 | R | + | + | + | − | − | + | IV | 99.9 | 1/1023 | M. porcinum | PAHs/crude oil/phenol |
A18 | Khorramabad (Lorestan) | Hospital water | 7.6 | 10 | 35 | S | − | − | + | + | + | − | I | 100 | 0/999 | M simiae | – |
A5 | Bushehr (Bushehr) | Sea sediment | 7.2 | 24 | 30 | R | + | + | + | − | − | + | IV | 100 | 0/872 | M. fredriksbergense | PAHs/crude oil |
A9 | Isfahan (Isfahan) | River sediment | 7.8 | 8 | 30 | R | + | + | + | − | − | + | IV | 100 | 0/872 | M. .fredriksbergense | PAHs/crude oil |
A8 | Shahin Shahr (Isfahan) | Oil refinery soil | 8.3 | 18 | 30 | R | + | − | + | − | − | − | IV | 99.4 | 4/663 | M. celeriflavum | PAHs |
A14 | Omidie (Khuzestan) | Oil well sediments | 6.9 | 20 | 25 | R | + | + | − | − | + | + | IV | 99.2 | 11/1378 | M. vaccae like | PAHs/crude oil |
A33 | Bandar anzali (Gilan) | River sediments | 6.8 | 20 | 35 | R | + | + | + | + | + | − | IV | 99.7 | 3/983 | M. novocastrense | Sulfate sodium |
A (24, A25, A52) | Isfahan (Isfahan) | Hospital water and soil | 7–8.8 | 12–28 | 35 | S | + | − | − | − | + | − | II | 99.6–99.9 | 1–4/995 | M. paragordonae | – |
A90 | Shahr Kurd (kohkiloye) | Hospital water | 7.6 | 14 | 35 | S | + | − | − | − | + | − | II | 99.9 | 1/995 | M. paragordonae | – |
A11 | Dorud (Lorestan) | Cement factory soil | 6.8 | 14 | 35 | R | + | − | + | + | + | + | IV | 100 | 0/1044 | M. aurum | PAHs/PVC/morpholine/piperidin |
A89 | Kermanshah (Kermanshah) | Petrochemical factory soil | 7 | 16 | 35 | R | + | − | + | + | + | + | IV | 100 | 0/1044 | M. aurum | PAHs/PVC/morpholine/piperidin |
A43 | Hamadan (Hamadan) | Power plant soil | 8.5 | 6 | 35 | R | + | − | − | + | + | + | IV | 99.12 | 11/1253 | M. neoaurum | PAHs/crude oil/pristan |
A27 | Kerman (Kerman) | Sarcheshmeh copper mine soil | 8.4 | 16 | 25 | R | − | + | + | − | + | − | IV | 99.63 | 4/1067 | M. obuense | PAHs/methoxy chloro ethane |
A85 | Chabahar (Sistan and balochestan) | Sea sediments | 7.4 | 14 | 35 | R | − | + | − | + | + | − | IV | 99.64 | 5/1385 | M. phocaicum like | PAHs/crude oil/fluoro-glycofen ethyl |
A3 | Ahwaz (Khuzestan) | River sediments | 7.8 | 16 | 35 | R | − | + | − | + | + | − | IV | 99.64 | 5/1385 | M. phocaicum like | PAHs/crude oil/fluoro-glycofen ethyl |
A (44, 49) | Khorramabad (Lorestan) | Forest soil | 7 –7.6 | 14–26 | 35 | R | − | − | − | + | − | − | IV | 98.56–100 | 0–12/1247 | M. fortuitum | Natural rubber/phenols/squalene |
A63 | Isfahan (Isfahan) | Hospital water | 7.4 | 16 | 35 | R | − | − | − | + | − | − | IV | 100 | 0/1247 | M. fortuitum | Natural rubber/phenols/squalene |
A (69, 91) | Najaf abad (Isfahan) | Tile factory sewage | 7.6 | 22 | 35 | R | − | − | − | + | − | − | IV | 98.56–100 | 0–12/1247 | M. fortuitum | Natural rubber/phenols/squalene |
A115 | Ramsar (Mazandaran) | Forest soil | 7 | 18 | 35 | R | − | − | − | + | − | − | IV | 98.88 | 6/1247 | M. fortuitum | Natural rubber/phenols/squalene |