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Fig. 2 | AMB Express

Fig. 2

From: Improving extracellular production of Serratia marcescens lytic polysaccharide monooxygenase CBP21 and Aeromonas veronii B565 chitinase Chi92 in Escherichia coli and their synergism

Fig. 2

Dose-response effects of CBP21 on the activity of Chi92 to colloidal chitin, α-chitin or β-chitin. 250 µl of different contents (0, 0.005, 0.05, 0.5, 5, 50, 500 nmol) of CBP21 and 250 µl of 1% substrate (colloidal chitin, α-chitin or β-chitin) in sodium phosphate buffer (50 mM, pH 7.0) were incubated in 37 °C for 2 h, then 250 μl of Chi92 (21 nmol) was added and these reaction mixtures were incubated in 37 °C for another 1 h. Sample were measured by the 3,5-dinitrosalicylic acid (DNS) assay. Each data value represents the average of three measurements

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