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Fig. 1 | AMB Express

Fig. 1

From: Improving extracellular production of Serratia marcescens lytic polysaccharide monooxygenase CBP21 and Aeromonas veronii B565 chitinase Chi92 in Escherichia coli and their synergism

Fig. 1

Comparison of the extracellular production of CBP21 or Chi92 fused with different signal peptides. Recombinant E. coli cells were constantly cultured for 24 h in TB medium with lactose-induction or in TB medium with 1% glycine addition after 12 h lactose-induction followed by another 12 h incubation. The intracellular (C) and extracellular (M) production of CBP21 (a) or Chi92 (b) fused with different signal peptides (as indicated in the top of each lane) were analysed by SDS-PAGE. CBP21S and Chi92S represent CBP21 and Chi92 with their native signal peptide, respectively. CBP21 and Chi92 represent CBP21 and Chi92 without any signal peptide, respectively. The red arrows indicate the CBP21s and Chi92s with molecular weight estimation of 20–25 or 91–96 kDa, respectively

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