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Table 3 The new pMMO primers designed based on aligned pmoC, A, and B compared to pmoA189

From: Survey of methanotrophic diversity in various ecosystems by degenerate methane monooxygenase gene primers

Primers Sequence MT %GC
PmoC374 5′-AGCARGACGGYACNTGGC-3′ 42,9 56
PmoA189 5′-GGNGACTGGGACTTCTGG-3′ 40,3 56
PmoA344 5′-ANGTCCAHCCCCAGAAGT-3′ 42,9 50
  1. MT melting temperature, %GC GC content in percentage