Table 3 The new pMMO primers designed based on aligned pmoC, A, and B compared to pmoA189
Primers | Sequence | MT | %GC |
|---|---|---|---|
PmoC374 | 5′-AGCARGACGGYACNTGGC-3′ | 42,9 | 56 |
PmoA189 | 5′-GGNGACTGGGACTTCTGG-3′ | 40,3 | 56 |
PmoA344 | 5′-ANGTCCAHCCCCAGAAGT-3′ | 42,9 | 50 |
Primers | Sequence | MT | %GC |
|---|---|---|---|
PmoC374 | 5′-AGCARGACGGYACNTGGC-3′ | 42,9 | 56 |
PmoA189 | 5′-GGNGACTGGGACTTCTGG-3′ | 40,3 | 56 |
PmoA344 | 5′-ANGTCCAHCCCCAGAAGT-3′ | 42,9 | 50 |