Table 2 Comparison of targeting ability between two newly designed degenerate primers and pmoA189
Phylum | pmoC374 | pmoA344 | pmoA189 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mismatches | Mismatches | Mismatches | ||||||||||
0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | |
Gammaproteobacteria | 10/18 | 18/18 | 18/18 | 18/18 | 16/18 | 18/18 | 18/18 | 18/18 | 7/18 | 18/18 | 18/18 | 18/18 |
Alphaproteobacteria | 16/16 | 16/16 | 16/16 | 16/16 | 14/16 | 16/16 | 16/16 | 16/16 | 16/16 | 16/16 | 16/16 | 16/16 |
Verrucomicrobia | 3/7 | 5/7 | 5/7 | 6/7 | 0/7 | 0/7 | 1/7 | 3/7 | 0/7 | 0/7 | 0/7 | 3/7 |
NC10 | 1/1 | 1/1 | 1/1 | 1/1 | 0/1 | 0/1 | 0/1 | 0/1 | 1/1 | 1/1 | 1/1 | 1/1 |