Fig. 5From: A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli Effect of the fermentation temperature profile on α-CGTase activity in the periplasmic space. Induction temperature was maintained at 25 °C (●), or performed using the two-stage temperature profile beginning at 25 °C and shifting to 30 °C at 32 h (■)Back to article page