Skip to main content
AMB Express
Fig. 4 | AMB Express

Fig. 4

From: A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli

Fig. 4

SDS-PAGE analysis of recombinant α-CGTase localization in E. coli. Escherichia coli cells had been induced at a constant 25 °C for 90 h (lanes 1, 3, 5, 7) or using the two-stage temperature control strategy of 25 °C for 32 h followed by 30 °C for 58 h (lanes 2, 4, 6, 8). M molecular weight standards, lanes 1 and 2 extracellular fractions, lanes 3 and 4 periplasmic fractions, lanes 5 and 6 insoluble inclusion bodies, lanes 7 and 8 soluble cytopiasmic fraction

Back to article page