Fig. 2

Effect of the temperature change time on extracellular α-CGTase production. Induction of α-CGTase expression was initiated at 25 °C. The induction temperature was shifted directly to 30 °C after 14 h (*), 24 h (▲), 28 h (♦), 32 h (■) or 36 h (×), of induction. The temperature of the control culture (●) was maintained at 25 °C. The growth curve of the control culture is shown as a dashed line