Table 1 Apparent kinetic constants of the recombinant DyP-type peroxidase from Pleurotus sapidus
Substrate | pH | Enzyme concentration (nM) | K m (µM) | k cat (s−1) | k cat K −1m (s−1 M−1) | v max (µM s−1) |
|---|---|---|---|---|---|---|
ABTS | 3.8 | 0.27 | 99 | 375 | 3.8 × 106 | 0.10 |
DMP | 4.5 | 1.8 | 1227 | 60 | 4.9 × 104 | 0.11 |
Guaiacol | 4.5 | 4.5 | 713 | 74 | 1.0 × 105 | 0.35 |
RBlue 5 | 4.0 | 5.4 | 24 | 18 | 7.5 × 105 | 0.10 |