Fig. 3

Cation-exchange chromatography as second purification step using an SP Sepharose FF column; ABTS oxidizing enzyme activity was detected in the area with a gray background; UV absorption in AU at 28 nm (blue line), detected activity (dashed line), buffer concentration (50 mM sodium acetate buffer pH 4.0 with 1 M NaCl) in  % (light gray line)