Skip to main content

Table 2 Effect of culture conditions for production of extracellular Keratinase from Arthrobacter sp. NFH5 in shake-flask cultivation

From: Production, partial optimization and characterization of keratinase enzyme by Arthrobacter sp. NFH5 isolated from soil samples

Culture condition Specific activity (U/ml) Relative activity (%)
Incubation period (h)
 24 25.8 100
 48 19.56 75.81
 72 15.02 58.22
 96 6.92 26.82
Incubation temperature (°C)
 28 4.52 19.72
 33 8.72 48.85
 37 17.85 100
 42 12.08 67.68
 47 6.32 35.41
 52 5.04 28.24
Initial pH
 5.0 5.08 20.56
 6.0 10.03 40.62
 7.0 24.69 100
 7.50 18.37 74.40
8.0 12.84 58.0
 9.0 9.48 38.39
 10.0 5.92 23.98
Inoculums volume (%, ml)
 2.0 2.08 11.06
 3.0 3.44 18.30
 4.0 10.26 54.60
 5.0 18.79 100
 6.0 9.65 51.35
 7.0 4.48 23.84
Agitation speed (rpm)
 120 8.72 32.98
 140 12.08 45.69
 150 26.44 100
 160 12.72 48.11
 180 6.16 23.30
  1. Data represent the means of triplicate