Table 2 Effect of culture conditions for production of extracellular Keratinase from Arthrobacter sp. NFH5 in shake-flask cultivation
Culture condition | Specific activity (U/ml) | Relative activity (%) |
|---|---|---|
Incubation period (h) | ||
24 | 25.8 | 100 |
48 | 19.56 | 75.81 |
72 | 15.02 | 58.22 |
96 | 6.92 | 26.82 |
Incubation temperature (°C) | ||
28 | 4.52 | 19.72 |
33 | 8.72 | 48.85 |
37 | 17.85 | 100 |
42 | 12.08 | 67.68 |
47 | 6.32 | 35.41 |
52 | 5.04 | 28.24 |
Initial pH | ||
5.0 | 5.08 | 20.56 |
6.0 | 10.03 | 40.62 |
7.0 | 24.69 | 100 |
7.50 | 18.37 | 74.40 |
8.0 | 12.84 | 58.0 |
9.0 | 9.48 | 38.39 |
10.0 | 5.92 | 23.98 |
Inoculums volume (%, ml) | ||
2.0 | 2.08 | 11.06 |
3.0 | 3.44 | 18.30 |
4.0 | 10.26 | 54.60 |
5.0 | 18.79 | 100 |
6.0 | 9.65 | 51.35 |
7.0 | 4.48 | 23.84 |
Agitation speed (rpm) | ||
120 | 8.72 | 32.98 |
140 | 12.08 | 45.69 |
150 | 26.44 | 100 |
160 | 12.72 | 48.11 |
180 | 6.16 | 23.30 |