|
Culture condition
|
Specific activity (U/ml)
|
Relative activity (%)
|
|---|
|
Incubation period (h)
|
|
24
|
25.8
|
100
|
|
48
|
19.56
|
75.81
|
|
72
|
15.02
|
58.22
|
|
96
|
6.92
|
26.82
|
|
Incubation temperature (°C)
|
|
28
|
4.52
|
19.72
|
|
33
|
8.72
|
48.85
|
|
37
|
17.85
|
100
|
|
42
|
12.08
|
67.68
|
|
47
|
6.32
|
35.41
|
|
52
|
5.04
|
28.24
|
|
Initial pH
|
|
5.0
|
5.08
|
20.56
|
|
6.0
|
10.03
|
40.62
|
|
7.0
|
24.69
|
100
|
|
7.50
|
18.37
|
74.40
|
|
8.0
|
12.84
|
58.0
|
|
9.0
|
9.48
|
38.39
|
|
10.0
|
5.92
|
23.98
|
|
Inoculums volume (%, ml)
|
|
2.0
|
2.08
|
11.06
|
|
3.0
|
3.44
|
18.30
|
|
4.0
|
10.26
|
54.60
|
|
5.0
|
18.79
|
100
|
|
6.0
|
9.65
|
51.35
|
|
7.0
|
4.48
|
23.84
|
|
Agitation speed (rpm)
|
|
120
|
8.72
|
32.98
|
|
140
|
12.08
|
45.69
|
|
150
|
26.44
|
100
|
|
160
|
12.72
|
48.11
|
|
180
|
6.16
|
23.30
|
- Data represent the means of triplicate
