Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation

Wei, Ya-Qin; Yang, Hong-Jian; Long, Rui-Jun; Wang, Zhi-Ye; Cao, Bin-Bin; Ren, Qin-Chang; Wu, Tian-Tian

doi:10.1186/s13568-017-0459-1

AMB Express

Table 2 End-products produced by the (Piromyces + M. ruminantium) Yak-G18 co-culture grown on chopped wheat straw, corn stalk, rice straw, Chinese wildrye and medicago sativa during the 7 days’ incubation

From: Characterization of natural co-cultures of Piromyces with Methanobrevibacter ruminantium from yaks grazing on the Qinghai-Tibetan Plateau: a microbial consortium with high potential in plant biomass degradation

End-products	(Piromyces + M. ruminantium) Yak-G18 co-culture
End-products	2 days	3 days	4 days	5 days	6 days	7 days	Average
Acetate (mM)
ws	9.23^d	12.36^c	20.56^b	23.10^b	24.13^d	23.36^c	18.79^a
cs	15.16^ab	22.24^ab	29.27^a	31.04^a	37.07^ab	39.60^a	29.07^ab
rs	11.34^cd	15.41^bc	20.34^b	24.55^b	30.23^c	32.01^b	22.31^c
cw	16.83^a	23.24^a	29.94^a	32.37^a	38.41^a	41.27^a	30.34^a
ms	13.50^bc	21.57^b	28.61^a	30.37^a	33.74^bc	36.94^ab	27.45^b
CH₄ (mmol/g DM)
ws	0.52^b	0.86^a	1.27^a	1.44^ab	2.33^a	2.35^a	1.46^a
cs	0.74^a	0.86^a	1.31^a	1.55^a	1.86^ab	1.90^b	1.37^b
rs	0.60^ab	0.96^a	1.16^a	0.93^c	1.42^bc	1.62^bc	1.11^c
cw	0.78^a	0.89^a	1.44^a	1.65^a	1.86^ab	1.96^b	1.43^ab
ms	0.61^ab	0.79^a	1.14^a	1.20^bc	1.30^c	1.28^c	1.05^c
d-lactate (mM)
ws	0.15^a	0.19^a	0.24^a	0.24^a	0.24^a	0.23^a	0.22^a
cs	0.12^a	0.15^a	0.16^b	0.16^b	0.16^a	0.17^ab	0.15^b
rs	0.14^a	0.16^a	0.19^ab	0.21^ab	0.21^a	0.22^a	0.19^ab
cw	0.12^a	0.14^a	0.13^b	0.16^b	0.16^a	0.15^b	0.14^b
ms	0.13^a	0.16^a	0.17^b	0.16^b	0.21^a	0.21^ab	0.17^ab
l-lactate (mM)
ws	3.86^a	4.41^a	4.98^a	5.22^ab	5.54^a	4.86^a	4.81^a
cs	3.92^a	4.32^ab	5.52^a	5.79^a	5.76^a	4.89^a	5.03^a
rs	3.26^a	3.26^b	3.50^b	3.56^b	3.29^b	3.57^a	3.40^b
cw	3.72^a	4.19^ab	5.42^a	5.73^a	5.63^a	4.79^a	4.91^a
ms	3.59^a	3.99^ab	5.19^a	5.46^ab	5.10^ab	4.55^a	4.64^a
Lactate (mM)
ws	4.02^a	4.60^a	5.21^a	5.45^ab	5.78^a	5.09^a	5.03^a
cs	4.04^a	4.47^a	5.68^a	5.93^a	5.93^a	5.06^a	5.19^a
rs	3.40^a	3.42^a	3.69^b	3.77^b	3.49^b	3.80^a	3.59^b
cw	3.84^a	4.32^a	5.55^a	6.01^a	5.79^a	4.94^a	5.05^a
ms	3.72^a	4.14^a	5.36^a	5.62^ab	5.31^ab	4.76^a	4.82^ab
Succinate (mM)
ws	0.02^a	0.04^ab	0.05^a	0.05^a	0.06^ab	0.06^ab	0.05^a
cs	0.03^a	0.04^ab	0.05^a	0.06^a	0.07^a	0.08^a	0.05^a
rs	0.03^a	0.03^b	0.04^a	0.05^a	0.05^b	0.05^b	0.04^a
cw	0.04^a	0.05^a	0.06^a	0.06^a	0.08^a	0.08^a	0.06^a
ms	0.03^a	0.03^b	0.05^a	0.07^a	0.08^a	0.08^a	0.06^a

Means in a row without same superscript letter differ as noted p value
SEM standard error of means (n = 3), ws wheat straw, cs corn stalk, rs rice straw, cw Chinese wildrye, ms medicago sativa

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