Table 1 Changes in free amino acid and ammonia concentrations of the 2 kDa membrane permeates of IYD supernatants

Ammonia

1.99 ± 0.37^a

3.48 ± 0.28^b

6.89 ± 0.33^c

2.32 ± 0.48^A

3.61 ± 0.31^B

6.09 ± 0.47^C

Aspartic acid

17.06 ± 2.33^b

4.38 ± 0.59^a

5.41 ± 0.63^a

16.30 ± 2.12^B

4.54 ± 0.57^A

4.41 ± 0.38^A

Serine

8.43 ± 0.83^b

4.68 ± 0.38^a

5.98 ± 0.32^a

7.74 ± 0.92^B

4.75 ± 0.41^A

4.71 ± 0.29^A*

Glutamic acid

20.91 ± 1.43^a

36.69 ± 1.41^b

75.64 ± 2.29^c

22.07 ± 2.99^A

34.01 ± 3.20^B

54.41 ± 2.70^C*

Glycine

4.59 ± 0.06^b

1.94 ± 0.25^a

1.77 ± 0.13^a

5.14 ± 1.00^B

2.58 ± 0.42^A

1.96 ± 0.30^A

Histidine

ND

ND

ND

ND

ND

ND

Arginine

1.10 ± 0.18^a

4.07 ± 0.54^b

5.96 ± 0.19^c

1.53 ± 0.19^A

3.44 ± 1.05^B

3.96 ± 0.70^B*

Threonine

4.16 ± 0.07^c

2.10 ± 0.20^b

1.05 ± 0.07^a

4.31 ± 0.59^C

2.08 ± 0.62^B

0.90 ± 0.14^A

Alanine

21.37 ± 1.64^a

22.32 ± 0.97^a

30.83 ± 0.81^b

18.85 ± 2.16^A

20.70 ± 1.55^A

21.97 ± 1.27^A*

Proline

4.79 ± 0.26^a

15.50 ± 1.87^b

18.88 ± 0.53^c

4.64 ± 0.77^A

13.71 ± 1.22^B*

13.70 ± 0.83^B*

Cysteine

ND

0.94 ± 0.04

ND

ND

0.95 ± 0.14

ND

Tyrosine

5.31 ± 0.07^c

1.66 ± 0.07^b

0.79 ± 0.06^a

4.97 ± 0.91^C

1.73 ± 0.23^B

0.61 ± 0.10^A

Valine

10.85 ± 0.31^b

5.11 ± 0.22^a

4.60 ± 0.09^a

10.53 ± 1.65^B

5.14 ± 0.52^A

3.60 ± 0.28^A*

Methionine

2.08 ± 0.08

ND

ND

1.94 ± 0.36

ND

ND

Lysine

5.83 ± 0.70^b

1.38 ± 0.15^a

0.40 ± 0.05^a

4.98 ± 0.86^B

1.03 ± 0.14^A

ND^*

Isoleucine

7.39 ± 0.16^b

1.58 ± 0.11^a

1.56 ± 0.09^a

7.23 ± 1.09^B

1.83 ± 0.24^A

1.25 ± 0.15^A*

Leucine

11.94 ± 0.29^c

2.87 ± 0.16^b

1.83 ± 0.08^a

11.46 ± 1.58^B

3.18 ± 0.38^A

1.57 ± 0.16^A

Phenylalanine

7.49 ± 0.26^c

1.65 ± 0.04^b

0.80 ± 0.03^a

7.25 ± 1.25^B

1.93 ± 0.24^A

0.76 ± 0.11^A

Tryptophan

ND

ND

ND

ND

ND

ND

Total

135.28 ± 7.52^b

109.43 ± 5.31^a

162.07 ± 4.68^c

131.25 ± 16.84^B

104.21 ± 10.32^A

119.44 ± 7.16^AB*