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Table 1 Screening of monoclonal antibodies against peptide-BSA conjugate (OD450)

From: Development of a sandwich enzyme-linked immunosorbent assay for dTMP-GH fusion protein by rational immunogen selection

Clone

Type

dTMP-GH

Peptide-BSA conjugate

TMP-GH

rhGH

BSA

1E10

IgG2b

1.857 ± 0.082

2.093 ± 0.094

0.073 ± 0.008

0.099 ± 0.007

0.072 ± 0.005

2C3

IgM

1.918 ± 0.097

1.711 ± 0.065

0.089 ± 0.006

0.091 ± 0.011

0.075 ± 0.005

2D3

IgM

2.166 ± 0.098

2.815 ± 0.085

0.081 ± 0.06

0.084 ± 0.008

0.118 ± 0.007

2E2

IgM

2.101 ± 0.096

1.758 ± 0.077

0.091 ± 0.07

0.082 ± 0.007

0.149 ± 0.009

3G2

IgM

2.245 ± 0.099

1.297 ± 0.067

0.071 ± 0.08

0.075 ± 0.008

0.068 ± 0.007

  1. Values are obtained from three independent experiments conducted in duplicate and shown as the mean ± SD