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Table 1 Bacterial strains and plasmids

From: Genetic evidence for a novel competence inhibitor in the industrially important Bacillus licheniformis

Strain or plasmid Relevant genotype Source or reference
Strains
 Escherichia coli DH5αF‘ endA1, hsdR17 (r K , m +K ), supE44, thi-1, gyrA96, relA1, ∆(lacIZYA-argF), U169, deoR, F’Φ(80dlacZ M15) Woodcock et al. (1989)
 Bacillus subtilis SCK6 BGSC 1A751 derivative, EmR, lacA::PxylA-comK Zhang and Zhang (2011)
 Bacillus licheniformis DSM13 Wild type DSMZ, Accession No. AE017333.1
 Bacillus subtilis NCIB3610 Wild type Branda et al. (2001)
 Bacillus licheniformis DSM13 ∆spoIV Sporulation-deficient DSM13 derivative Hoffmann et al. (2010)
 Bacillus licheniformis MW3.1 DSM13 derivative; ΔhsdR1, ΔhsdR2, ΔpyrE Hoffmann et al. (2010)
 Bacillus licheniformis CM1 MW3.1 ∆comI::aphA This work
 Bacillus licheniformis CM2 MW3.1 P comI ::pMUTIN-comI This work
Plasmids
 pMMcomK E. coli/Bacillus shuttle vector, pMM1522 derivative, B. licheniformis MW3 ComK expression vector, AmpR, TetR Hoffmann et al. (2010)
 pUppem pUCBM20 derivative, PrpoB-upp fusion, AmpR, EmR, ori E. coli Borgmeier et al. (2012)
 pMB03 pUppem derivative, KanR, EmR, AmpR, ori E.coli M. Buchholz, this laboratory
 pUE∆comI pUppem derivative, ∆comI::aphA substitution cassette This work
 pMUTIN-GFP+ pMUTIN derivative,integrative vector for Bacillus, AmpR, EmR Kaltwasser et al. (2002)
 pMUTIN-comI pMUTIN-GFP+ derivative, P comI -GFP fusion, inducible (IPTG) comI expression This work