Genetic evidence for a novel competence inhibitor in the industrially important Bacillus licheniformis

AMB Express

Table 1 Bacterial strains and plasmids

Strain or plasmid	Relevant genotype	Source or reference
Strains
Escherichia coli DH5αF‘	endA1, hsdR17 (r ⁻_K , m ⁺_K ), supE44, thi-1, gyrA96, relA1, ∆(lacIZYA-argF), U169, deoR, F’Φ(80dlacZ M15)	Woodcock et al. (1989)
Bacillus subtilis SCK6	BGSC 1A751 derivative, Em^R, lacA::PxylA-comK	Zhang and Zhang (2011)
Bacillus licheniformis DSM13	Wild type	DSMZ, Accession No. AE017333.1
Bacillus subtilis NCIB3610	Wild type	Branda et al. (2001)
Bacillus licheniformis DSM13 ∆spoIV	Sporulation-deficient DSM13 derivative	Hoffmann et al. (2010)
Bacillus licheniformis MW3.1	DSM13 derivative; ΔhsdR1, ΔhsdR2, ΔpyrE	Hoffmann et al. (2010)
Bacillus licheniformis CM1	MW3.1 ∆comI::aphA	This work
Bacillus licheniformis CM2	MW3.1 P_comI::pMUTIN-comI	This work
Plasmids
pMMcomK	E. coli/Bacillus shuttle vector, pMM1522 derivative, B. licheniformis MW3 ComK expression vector, Amp^R, Tet^R	Hoffmann et al. (2010)
pUppem	pUCBM20 derivative, PrpoB-upp fusion, Amp^R, Em^R, ori_{E. coli}	Borgmeier et al. (2012)
pMB03	pUppem derivative, Kan^R, Em^R, Amp^R, ori_E.coli	M. Buchholz, this laboratory
pUE∆comI	pUppem derivative, ∆comI::aphA substitution cassette	This work
pMUTIN-GFP+	pMUTIN derivative,integrative vector for Bacillus, Amp^R, Em^R	Kaltwasser et al. (2002)
pMUTIN-comI	pMUTIN-GFP+ derivative, P_comI-GFP fusion, inducible (IPTG) comI expression	This work