Effect of Eudragit S100 nanoparticles and alginate chitosan encapsulation on the viability of Lactobacillus acidophilus and Lactobacillus rhamnosus

AMB Express

Table 1 Cell survivability (CFU/ml) of three forms of Lactobacillus acidophilus and Lactobacillus rhamnosus (free, mono layer and double layer) after treatment in simulated gastric juice during 120 min (with 30 min interval) and then treatment in simulated intestinal juice with bile salt for 150 min (mean of log count ± SD)

Bacteria	Form	0 min	30 min	60 min	90 min	120 min
Lactobacillus acidophilus	Fa	9.78 ± 0.09	7.27 ± 0.01	5.53 ± 0.14	4.56 ± 0.12	3.00 ± 0.57
	M1b	9.52 ± 0.00	8.17 ± 0.01	7.543 ± 0.02	6.60 ± 0.00	5.02 ± 0.03
	M2b	8.61 ± 0.15	8.28 ± 0.02	7.77 ± 0.29	7.70 ± 0.07	6.02 ± 0.33
Lactobacillus rhamnosus	Fa	9.65 ± 0.31	6.27 ± 0.01	5.45 ± 0.43	3.33 ± 0.14	3.11 ± 0.30
	M1b	9.31 ± 0.15	8.48 ± 0.01	6.43 ± 0.00	5.10 ± 0.02	4.82 ± 0.00
	M2c	9.93 ± 0.04	9.05 ± 0.49	8.87 ± 0.17	8.23 ± 0.00	7.87 ± 0.26

F free form, M1 encapsulated form only with chitosan coating (single coating), M2 encapsulated form with chitosan and Eudragit nanoparticles coating (double coating)
a, b, c The different lower case letters indicate statistically significant difference between declines in bacterial count between different bacterial form (statistical analysis was performed separately for each bacterial species)