Fig. 6
From: Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum
Assignment of sigma factors to the P2sigA promoter. a Sequence of the P2sigA promoter (Halgasova et al. 2001). b In vitro transcription with P2sigA as a template. Individual sigma factors associated with RNAP are shown at the top. The specific transcripts are indicated with an arrow. c P2sigA promoter activity determined with the two-plasmid system in C. glutamicum WT by measuring the fluorescence intensity of the Gfpuv reporter. Ft − F0 is the difference between the fluorescence at the sampling time (3, 6, 24 h) and the fluorescence at time 0 (before sigma gene induction). The sigma factors whose genes were overexpressed are indicated at the bottom. The SDs of three measurements are depicted as error bars