Fig. 5

Assignment of a sigma factor to the Pcmt1 promoter. a Sequence of the cmt1 promoter region. b In vitro transcription with Pcmt1 as a template. Individual sigma factors associated with RNAP are shown at the top. N no sigma factor was added to the RNAP core. The specific transcript is indicated with an arrow. c Pcmt1 promoter activity determined with the two-plasmid system in C. glutamicum WT by measuring the fluorescence intensity of the Gfpuv reporter. The sigma factors whose genes were overexpressed are indicated at the bottom. IPTG was added at time 0. AU arbitrary units. The SDs of three measurements are depicted as error bars