Fig. 4

Assignment of a sigma factor to the Pcg2556 promoter. a Sequence of the cg2556 (cgR_2208) promoter (Toyoda and Inui 2016a). The −35 and −10 sequence elements are in bold, the transcriptional start site is underlined. b In vitro transcription with Pcg2556 as a template. Individual sigma factors associated with RNAP are shown at the top. N no sigma was added to the RNAP core. The specific transcript is indicated with an arrow. c Pcg2556 activity determined with the two-plasmid system in C. glutamicum WT by measuring the fluorescence intensity of the Gfpuv reporter. The sigma factors whose genes were overexpressed are indicated at the bottom. The SDs of three measurements are depicted as error bars