Fig. 3

Assignment of sigma factors to the PsigB promoter. a Sequence of the PsigB promoter (Halgasova et al. 2001). The assumed −35 and −10 sequence elements are in bold, the transcriptional start site is underlined. b In vitro transcription with PsigB as a template. Individual sigma factors associated with RNAP are shown at the top. N no sigma factor protein was added to the RNAP core. The specific transcripts are indicated with an arrow. c PsigB promoter activity determined using the two-plasmid C. glutamicum ∆sigH by measuring the fluorescence intensity of the Gfpuv reporter. The sigma factors whose genes were overexpressed are indicated at the bottom. IPTG was added at time 0. AU arbitrary units. The SDs of three measurements are depicted as error bars