Fig. 2

Assignment of a sigma factor to the PtrxB1 promoter. a Sequence of the PtrxB1 promoter (Nakunst et al. 2007). The −35 and −10 sequence elements are in bold, the transcriptional start site is underlined. b In vitro transcription with PtrxB1 as a template. Individual sigma factors associated with RNAP are shown at the top. N no sigma factor was added to the RNAP core. The specific transcript is indicated with an arrow. c PtrxB1 activity determined with the two-plasmid system in C. glutamicum ∆sigH by measuring the fluorescence intensity of the Gfpuv reporter. The sigma factors whose genes were overexpressed are indicated at the bottom. IPTG was added at time 0. d PtrxB1 activity in C. glutamicum ∆sigH, ∆sigM or ∆sigE strains carrying a single plasmid, pEPR-PtrxB1. The values for the WT strain carrying the empty vector pEPR1 are shown as a control. AU arbitrary units. The SDs of three measurements are depicted as error bars