Fig. 1

Assignment of a sigma factor to the PrshA promoter. a Sequence of the rshA (cg0876) promoter (Pfeiffer-Sancar et al. 2013). The −35 and −10 sequence elements are in bold, the transcriptional start site is underlined. b In vitro transcription with PrshA as a template. Individual sigma factors associated with RNAP are shown at the top. N no sigma factor protein was added to the RNAP core. The specific transcript is indicated with an arrow. c PrshA activity determined with the two-plasmid system in C. glutamicum ∆sigH by measuring the fluorescence intensity of the Gfpuv reporter. The sigma factors whose genes were overexpressed are indicated at the bottom. IPTG was added at time 0. AU arbitrary units. The standard deviations (SDs) of three measurements are depicted as error bars