Comparison of duplex PCR and phenotypic analysis in differentiating Candida dubliniensis from Candida albicans from oral samples

AMB Express

Table 1 The primer sequences used for amplification of C. albicans and C. dubliniensis for duplex PCR

Target gene	Target species	Primer	Primer sequence (5′–>3′)	Amplicon size (bp)
ITS 2	C. albicans	CALF	TGG TAA GGC GGG ATC GCT T	100
ITS 2	C. albicans	CALR	GGT CAA AGT TTG AAG ATA TAC	100
ITS 1	C. dubliniensis	CDLF	AAA CTT GTC ACG AGA TTA TTT TT	325
ITS 2	C. dubliniensis	CDLR	AAA GTT TGA AGA ATA AAA TGG C	325