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Table 4 The effect of medium supplemented with horse hemolysate, hose serum, and hemin on the MIC of the Natto peptide with S. pneumoniae R6 strain

From: Novel antimicrobial activities of a peptide derived from a Japanese soybean fermented food, Natto, against Streptococcus pneumoniae and Bacillus subtilis group strains

Supplement Concentrationb MIC (μg/mL)
None >128
Horse hemolysatea 5% 8
  1% 4
  0.32% 4
  0.10% 8
  0.032% 32
  0.010% 64
  0.0032% 128
  0.0010% >128
Supernatant of heat-treated horse hemolysate 20% 4
  5% 32
Horse serum 5% >128
Hemina 6.9 μg/mL 8
  3.4 μg/mL 32
  1.7 μg/mL 64
  0.86 μg/mL 128
  0.43 μg/mL >128
  1. aHose hemolysate [5%(v/v)] and hemin (6.9 μg/mL) did not any antimicrobial effects against S. pneumoniae
  2. bThe percent concentrations are (v/v)