Fig. 4

Survival curves of B. subtilis strain in the presence and absence of the Natto peptide. a B. subtilis AHU 1708^T cells were pre-cultured to a cell density of approximately 10⁷ cells/mL in TH broth containing 5% (v/v) horse hemolysate. The Natto peptide was then added at a concentration of 64 μg/mL. b B. subtilis AHU 1708^T cells were suspended at a cell density of approximately 10⁵ cells/mL in TH broth containing 5% (v/v) horse hemolysate. The Natto peptide was then added at a concentration of 64 μg/mL, and the cells were cultured. Throughout the cultivation, the cultures were sampled, and culture aliquots were spread on TH agar supplemented with 5% (v/v) horse hemolysate, and cultured for 24 h. Grown colonies were then counted. Dashed lines indicate cell numbers in the presence of the Natto peptide. Solid lines indicate cell numbers in the absence of the Natto peptide. Each experiment performed at least three times, and the representative results are presented. The similar results were observed in the same experiments