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Table 1 HPLC analysis of FOS yield and composition produced by the FFase of A. tubingensis XG21 at different substrate concentrations

From: A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production

Substrate (w/v) (%) Sucrose (%) Glucose (%) Fructose (%) GF2 (%)b GF3 (%)b GF4 (%)b GFn (%)
0.2 5.4 (±0.2)a 49.2 (±2.3) 44.5 (±3.1) 1.0 (±0.2) 0.0 (±0.0) 0.0 (±0.0) 1.0
2.0 16.5 (±2.6) 44.9 (±1.2) 24.9 (±4.2) 8.3 (±0.3) 4.9 (±0.3) 0.5 (±0.1) 13.7
5.0 21.0 (±1.7) 40.8 (±2.1) 17.6 (±1.3) 10.2 (±1.2) 8.6 (±0.8) 2.9 (±0.2) 21.7
20.0 18.5 (±3.2) 37.9 (±1.9) 5.3 (±0.6) 13.9 (±0.9) 19.1 (±2.2) 5.4 (±1.2) 38.4
50.0 8.2 (±1.2) 31.3 (±3.2) 1.7 (±0.2) 21.8 (±2.2) 30.5 (±2.3) 6.6 (±1.7) 56.9
  1. The enzyme dosage was 6 U/g sucrose (substrate) and the reaction was conducted at 50 °C for 8 h
  2. a Data are the mean of three independent experiments; values in parentheses show standard deviations
  3. b GF2 kestose; GF3 nystose; GF4 fructofuranosyl nystose