Table 1 HPLC analysis of FOS yield and composition produced by the FFase of A. tubingensis XG21 at different substrate concentrations
Substrate (w/v) (%) | Sucrose (%) | Glucose (%) | Fructose (%) | GF2 (%)b | GF3 (%)b | GF4 (%)b | GFn (%) |
|---|---|---|---|---|---|---|---|
0.2 | 5.4 (±0.2)a | 49.2 (±2.3) | 44.5 (±3.1) | 1.0 (±0.2) | 0.0 (±0.0) | 0.0 (±0.0) | 1.0 |
2.0 | 16.5 (±2.6) | 44.9 (±1.2) | 24.9 (±4.2) | 8.3 (±0.3) | 4.9 (±0.3) | 0.5 (±0.1) | 13.7 |
5.0 | 21.0 (±1.7) | 40.8 (±2.1) | 17.6 (±1.3) | 10.2 (±1.2) | 8.6 (±0.8) | 2.9 (±0.2) | 21.7 |
20.0 | 18.5 (±3.2) | 37.9 (±1.9) | 5.3 (±0.6) | 13.9 (±0.9) | 19.1 (±2.2) | 5.4 (±1.2) | 38.4 |
50.0 | 8.2 (±1.2) | 31.3 (±3.2) | 1.7 (±0.2) | 21.8 (±2.2) | 30.5 (±2.3) | 6.6 (±1.7) | 56.9 |