E. coli
|
SCS110
|
rpsL (Strr) thr leu endA thi-l lacY galK galT ara tonA tsx dam dcm
|
Stratagene
|
supE44Δ (lac-proAB) [F’traD36 proAB lacl
q
ZΔM15]
| |
Novablue
|
endA1 hsdR17 (rK
−
mK
+) supE44 thi-1 gyrA96 relA1 lac recA1/F’
|
Novagen
|
[proAB
+
lac I
q
Z ΔM15 Tn10(tet
r)]
| |
C. efficiens
|
NBRC 100395
|
Wild-type C. efficiens, YS314
|
NBRC
|
C. glutamicum
|
ATCC 13032
|
Wild-type C. glutamicum, biotin-auxotrophic, l-glutamate producing strain
|
ATCC
|
ATCC 21420
|
C. glutamicum, l-phenylalanine producing strain
|
ATCC
|
F
|
C. glutamicum ATCC 21420 derivative harboring pCH
|
Okai et al. (2016)
|
FVan
|
C. glutamicum ATCC 21420 derivative harboring pCH-vanABce
|
This study
|
Plasmids
|
pCH
|
E. coli-C. glutamicum shuttle vector with HCE promoter, Kmr
|
Tateno et al. (2007)
|
pCH-vanABce
|
pCH containing CE0634-CE0635 (vanAB) from C. efficiens NBRC 100395
|
This study
|
Oligonucleotide primers
|
CE0634_up300-F
|
tcattgaccgagtactccgttt
| |
CE0635_R
|
tcagggcacgtcaattgtcaggtggatatt
| |
BglI-CE0634_up300-F
|
CAACAGTTGCGCAGCCTGAATGGCtcattgaccgagtactccgttt
| |
BglI-CE0635_R
|
ATAAATCGCATTCGCCATTCAGGCTGATCGCTATTGCCCTCCGATTATTAGGAGGGCGATtcagggcacgtcaattgtcaggtggatatt
| |