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Table 4 Central composite design matrix of the independent variables with the corresponding experimental values of succinic acid concentration

From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.

Run Crude glycerol (x1) MgCO3 (x2) K2HPO4 (X3) Succinic acid (g/L)
1 12.5 12.5 0.5 7.95 ± 0.08
2 27.5 12.5 0.5 14.2 ± 0.19
3 12.5 27.5 0.5 13.15 ± 0.03
4 27.5 27.5 0.5 20.27 ± 0.6
5 12.5 12.5 1.5 8.26 ± 0.13
6 27.5 12.5 1.5 14.2 ± 0.02
7 12.5 27.5 1.5 13.29 ± 0.6
8 27.5 27.5 1.5 19.75 ± 0.45
9 7.4 20 1 10.13 ± 0.01
10 32.6 20 1 17.62 ± 0.67
11 20 7.4 1 9.6 ± 0.2
12 20 32.6 1 16.09 ± 0.45
13 20 20 0.16 15.42 ± 0.24
14 20 20 1.84 16.08 ± 0.56
15 20 20 1 16.38 ± 0.13
16 20 20 1 16.19 ± 0.38
17 20 20 1 16.68 ± 0.28
18 20 20 1 16.29 ± 0.09
19 20 20 1 15.98 ± 0.19
20 20 20 1 15.99 ± 0.33