Table 4 Central composite design matrix of the independent variables with the corresponding experimental values of succinic acid concentration
From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.
Run | Crude glycerol (x1) | MgCO3 (x2) | K2HPO4 (X3) | Succinic acid (g/L) |
|---|---|---|---|---|
1 | 12.5 | 12.5 | 0.5 | 7.95 ± 0.08 |
2 | 27.5 | 12.5 | 0.5 | 14.2 ± 0.19 |
3 | 12.5 | 27.5 | 0.5 | 13.15 ± 0.03 |
4 | 27.5 | 27.5 | 0.5 | 20.27 ± 0.6 |
5 | 12.5 | 12.5 | 1.5 | 8.26 ± 0.13 |
6 | 27.5 | 12.5 | 1.5 | 14.2 ± 0.02 |
7 | 12.5 | 27.5 | 1.5 | 13.29 ± 0.6 |
8 | 27.5 | 27.5 | 1.5 | 19.75 ± 0.45 |
9 | 7.4 | 20 | 1 | 10.13 ± 0.01 |
10 | 32.6 | 20 | 1 | 17.62 ± 0.67 |
11 | 20 | 7.4 | 1 | 9.6 ± 0.2 |
12 | 20 | 32.6 | 1 | 16.09 ± 0.45 |
13 | 20 | 20 | 0.16 | 15.42 ± 0.24 |
14 | 20 | 20 | 1.84 | 16.08 ± 0.56 |
15 | 20 | 20 | 1 | 16.38 ± 0.13 |
16 | 20 | 20 | 1 | 16.19 ± 0.38 |
17 | 20 | 20 | 1 | 16.68 ± 0.28 |
18 | 20 | 20 | 1 | 16.29 ± 0.09 |
19 | 20 | 20 | 1 | 15.98 ± 0.19 |
20 | 20 | 20 | 1 | 15.99 ± 0.33 |