Table 3 Plackett–Burman design for eight variables with the corresponding experimental values of succinic acid concentration
From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.
Run | Crude glycerol | Whey permeate | Urea | MgCO3 | K2HPO4 | MgSO4 × 7H2O | CaCl2 | NaCl | D1 | D2 | D3 | Succinic acid (g/L) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | 2.42 ± 0.22 |
2 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | 4.87 ± 0.67 |
3 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | 3.63 ± 0.13 |
4 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | nd |
5 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | 9.42 ± 0.15 |
6 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | nd |
7 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | 1.76 ± 0.34 |
8 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | 4.29 ± 0.12 |
9 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | 3.45 ± 0.16 |
10 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | 3.17 ± 0.45 |
11 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | 6.35 ± 0.02 |
12 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | nd |