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Table 3 Plackett–Burman design for eight variables with the corresponding experimental values of succinic acid concentration

From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.

Run Crude glycerol Whey permeate Urea MgCO3 K2HPO4 MgSO4 × 7H2O CaCl2 NaCl D1 D2 D3 Succinic acid (g/L)
1 +1 −1 −1 −1 +1 −1 +1 +1 −1 +1 +1 2.42 ± 0.22
2 −1 +1 −1 +1 +1 −1 +1 +1 +1 −1 −1 4.87 ± 0.67
3 −1 +1 +1 −1 +1 +1 +1 −1 −1 −1 +1 3.63 ± 0.13
4 −1 −1 −1 +1 −1 +1 +1 −1 +1 +1 +1 nd
5 +1 −1 +1 +1 +1 −1 −1 −1 +1 −1 +1 9.42 ± 0.15
6 −1 −1 +1 −1 +1 +1 −1 +1 +1 +1 −1 nd
7 +1 +1 −1 −1 −1 +1 −1 +1 +1 −1 +1 1.76 ± 0.34
8 −1 +1 +1 +1 −1 −1 −1 +1 −1 +1 +1 4.29 ± 0.12
9 +1 +1 +1 −1 −1 −1 +1 −1 +1 +1 −1 3.45 ± 0.16
10 +1 −1 +1 +1 −1 +1 +1 +1 −1 −1 −1 3.17 ± 0.45
11 +1 +1 −1 +1 +1 +1 −1 −1 −1 +1 −1 6.35 ± 0.02
12 −1 −1 −1 −1 −1 −1 −1 −1 −1 −1 −1 nd