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Table 1 Plackett–Burman design for seven variables with the corresponding experimental values of biomass concentration

From: Media optimization for economic succinic acid production by Enterobacter sp. LU1.

Run Crude glycerol Urea MgCO3 MgSO4 × 7H2O CaCl2 K2HPO4 NaCl Biomass (g/L)
1 −1 −1 −1 +1 +1 +1 −1 1.10 ± 0.02
2 +1 −1 −1 −1 −1 +1 +1 1.48 ± 0.01
3 −1 +1 −1 −1 +1 −1 +1 1.32 ± 0.03
4 +1 +1 −1 +1 −1 −1 −1 4.63 ± 0.1
5 −1 −1 +1 +1 −1 −1 +1 1.08 ± 0.01
6 +1 −1 +1 −1 +1 −1 −1 1.45 ± 0.01
7 −1 +1 +1 −1 −1 +1 −1 1.42 ± 0.04
8 +1 +1 +1 +1 +1 +1 +1 8.03 ± 0.22