Fig. 2

Deletion of Lsp protein genes individually or in various combinations in Lysobacter enzymogenes did not disrupt twitching motility (a) and production of extracellular protease, cellulase and chitinase (b). In a, individual cells or cell clusters produced at the margin of colony are characteristic of twitching motility in L. enzymogenes. OH11: wild-type strain of L. enzymogenes; ΔpilA: twitching-motility deficient mutant used as a negative control; Δlsp1, Δlsp2 and Δlsp3: mutants with in-frame deletions lsp1, lsp2 and lsp3, respectively; Δlsp12, Δlsp13 and Δlsp23: double in-frame deletion mutants with double deletions at lsp1 and lsp2, lsp1 and lsp3, and lsp2 and lsp3, respectively; Δlsp123: the triple deletion mutant lacking lsp1, lsp2 and lsp3