|
Via
|
Enzyme
|
Recovery (%)
|
Purification fold
|
References
|
---|
Ni Sepharose purification
|
Ni Sepharose beads
|
Hyd
|
11.2
|
23.7
|
This study
|
IMAC chromatography
|
Hyd
|
16.6
|
22.6
|
Xu et al. (2003)
|
Ni Sepharose beads
|
Carb
|
67.5
|
50.9
|
This study
|
IMAC chromatography
|
Carb
|
70.0
|
–
|
Chen et al. (2003)
|
Functionalized magnetic bead purification
|
Functionalized magnetic beads
|
Hyd
|
11.9
|
35.9
|
This study
|
IMAMa
|
Hyd
|
3.5
|
6.8
|
Ko et al. (2011)
|
Functionalized magnetic beads
|
Carb
|
32.5
|
126.6
|
This study
|
IMAC chromatography
|
Carb
|
55.0
|
11.3
|
Pietzsch et al. (2000)
|
- Values for the hydantoinase and carbamoylase determined in this work (italics) compared to values reported in literature using other hydantoinases and carbamoylases (regular). Ni Sepharose purification: elution 1 + elution 2
-
aImmobilized metal ion affinity membrane