Table 4 Overview of investigated purification and immobilization methods for both enzymes and comparison to other works
Via | Enzyme | Recovery (%) | Purification fold | References | |
|---|---|---|---|---|---|
Ni Sepharose purification | Ni Sepharose beads | Hyd | 11.2 | 23.7 | This study |
IMAC chromatography | Hyd | 16.6 | 22.6 | Xu et al. (2003) | |
Ni Sepharose beads | Carb | 67.5 | 50.9 | This study | |
IMAC chromatography | Carb | 70.0 | – | Chen et al. (2003) | |
Functionalized magnetic bead purification | Functionalized magnetic beads | Hyd | 11.9 | 35.9 | This study |
IMAMa | Hyd | 3.5 | 6.8 | Ko et al. (2011) | |
Functionalized magnetic beads | Carb | 32.5 | 126.6 | This study | |
IMAC chromatography | Carb | 55.0 | 11.3 | Pietzsch et al. (2000) |